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Image Search Results
Journal: Molecular Therapy
Article Title: CDK1-loaded extracellular vesicles promote cell cycle to reverse impaired wound healing in diabetic obese mice
doi: 10.1016/j.ymthe.2025.01.039
Figure Lengend Snippet: Characterization of CDK1-loaded sEVs (A) Schematic of engineering CDK1-loaded sEVs. (B) Determination of EV concentration of mock (empty vector) and CDK1-loaded sEVs ( n = 7 each). (C) Size distribution of sEVs ( n = 7). (D) Representative vFC analyses of sEVs ( n = 7). MFI, mean fluorescent intensity. (E) Immunoblotting of sEVs using EV markers, CDK1 and calnexin. (F) Normalized CDK1 expression based on immunoblotting, region of interest values shown ( n = 4; ∗∗ p < 0.01). (G) Immunoblotting of sEVs using phosphorylation of CDK1 Tyr15 vs. pan-CDK1, and Alix as EV marker.
Article Snippet: The flow cytometer was calibrated for vesicle size using
Techniques: Concentration Assay, Plasmid Preparation, Western Blot, Expressing, Phospho-proteomics, Marker